Not sure about the on line purchase but I know that the lines in front of the Uffizi are constant and in season. These lines are filed with people that hold tickets, I know being that I stud in line with a ticket. Purchasing on line will not grant one privilege to go in ahead of others. Special tours and groups do get in sooner but not all. Being that it is October that you plan to go, I do not think you will have the crowds. It is in the New Year the tourist season picks up. I personally prefer the Pitti exhibit but all are worth the time even the small churches around town. Enjoy.
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